Innovation Sphere


  • Hendrik Viljoen



Project TitleExpedited PCR with Stirring and the Use of Super Paramagnetic Nanoparticles (SPNPs) to Concentrate DNA in Lysate
Track Code2016-017
Short Description

This is a method for dramatically expediting PCR. The use of SPNPs facilitates transport of DNA, on a metal stirring rod, from lysis to PCR, decreasing the risk of contamination. 

Tagsresearch tools, diagnostics, expedited PCR, Super Paramagnetic Nano Particles (SPNPs), Isothermal PCR
Posted DateFeb 17, 2017 3:11 PM


Hendrik Viljoen

Technology Description

This innovative technology dramatically accelerates and increases the yield of isothermal PCR reactions by constant stirring during the reaction. By utilizing SPNPs, and a metal stirring rod, this device can also serve two additional functions. First, as a porting platform for DNA from a lysis cuvette to a PCR cuvette, and second, to immobilize SPNPs on the rod while the contents of the cuvette are exchanged with the PCR mixture.

Inventive Feature(s)

•  Transportation of DNA from lysis cuvette to PCR cuvette utilizing a metal stirring rod and SPNPs
•  A drill bit inserted into the cuvette and rotated to induce mixing in the PCR mixture


•  Average system performance 5x faster than a non-stirred signal
•  Higher signal strength than a non-stirred signal  
•  Limits contamination when used as a porter for DNA to and from amplification with PCR

Potential Application(s)

•  Rapid PCR diagnostics in healthcare, agriculture and environmental settings 
•  Suitable for using at a patient's point of care setting for healthcare applications or field use for agricultural and environmental applications

Intellectual Property

Patent application filed


Related Technologies

Licensing Contact

Arpi Siyahian, Ph.D.
Technology Manager
Phone: 818-599-2543

About NUtech Ventures

NUtech Ventures is the non-profit technology commercialization affiliate of the University of Nebraska—Lincoln. Our mission is to facilitate the commercialization and practical use of innovations generated through the research activities at the University of Nebraska.